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Angry relatives lashed out at health bosses at Calderdale Royal in Halifax after loved ones died of the superbug Clostridium difficile.
A government report this week said hospitals are failing to stop the bug, which kills 1,000 people a year.
Liz Greenwood, whose father Leslie Slater, 79, died after bladder stone surgery, claimed conditions at Calderdale Royal in Halifax were "disgusting" and that staff ignored signs that he had the bug.
Calderdale and Huddersfield NHS Trust said: "The concerns have been thoroughly investigated and a letter explaining the conclusions sent to the family”.
Relatives of Patrick Martin, 72, who died in March after a cancer operation, wants a public inquiry.
Daughter Jacqueline Johnson said: "The NHS cannot confirm or deny he had the deadly strain because they never tested him”.
Elaine Maxwell, nursing director at Oldchurch Hospital in Romford, Essex, said two cases of the same strain were found later "but at the time we were unaware any such strain existed".
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